Converting 2D CZI images into 3D Stack with MicroFile+

Q: I am currently trying to use Microfile+ to convert .czi images to .jpx files using “combine 2D images into 3D stack”, but whenever I start the conversion it gets stuck on “starting conversion” and loads forever. Is there something I am doing wrong?

Dear User,

My first suggestion would be to try converting the CZI 2D images to .jp2 2D images first. Once the 2D images are all in the .jp2 format, I recommend using the Combine 2D Images into 3D Stacks option to compile the images into a .jpx stack. CZI images can be container files, meaning they can hold many images in one file. Though Microfile+ can export each of these images to separate files, I do not think it is designed to work out how to combine those subimages to create a stack.

I hope this helps!

Aidan