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Discussion about this site, its organization, how it works, and how we can improve it.

Neuron Reconstruction

This category is for questions related to neuron tracing and reconstruction for Neurolucida and Neurolucida 360 software users.


This category is for discussing basic and advanced concepts of stereology, and our Stereo Investigator tools for conducting stereology.

Image and Data Management

This category is for asking and discussing questions about image and data management and standardization.

Brain Reconstruction and Mapping

This category is for asking questions about and discussing brain volume reconstruction, brain-wide measurements, and mapping data to atlases.


Ask questions and get guidance on most light microscopy techniques and sample preparation for neuron tracing, stereology, and brain mapping.

Multi-Photon Imaging

This category is for questions related to ScanImage and multi-photon imaging.

Vascular Reconstruction

This category is for question related to vascular reconstruction.


This category is for asking and discussing questions about C. elegans imaging and behavioral analysis.

Virtual Microscopy for Education

This category is for asking questions about virtual microscopy for medical education and MBF’s Biolucida virtual microscopy platform.

Neuromorphological File Specification

Ask questions related to Neuromorphological file format.
MBF Bioscience has chosen to openly document their digital reconstruction file format, Neuromorphological File Specification (4.0), available at (Angstman et al. 2020). The structures represented have been continually developed for over 30 years, balancing accurate 3D representation of microscopic structures with the efficiency for reading, writing, storing, and rendering the reconstruction data. The neuromorphological file format balances structure with flexibility by storing each modeled object as a unique data element, and providing mechanisms for grouping any number and type of data elements. File-level metadata is retained to provide detail on the origin of the sample, ensuring that the provenance of derivative data is tracked and that important source information is not separated from the corresponding data. The decision to implement each neuromorphological structure, from spines to varicosities, was influenced by top neuroscientists who sought to maintain a meaningful model and have the necessary analysis options for that entity. The file specification has evolved with academia and discovery, and will continue to be updated as needed to define added or modified data elements.

Announcement and News

Keep up with official MBF Bioscience news, releases, and important announcements.


This category informs you about our upcoming and past webinars. We will also be answering questions that you have related to the webinars.