3D Printing plans for both 10 and 20 well chambers are available for download at: https://mbfbioscience.sharefile.com/d-sceebc7e4ac07465da284b8749c436507
Materials: Be sure to use an acrylic like substrate, we used Veroclear (OBJ-03271) by Stratasys and we print on an Eden 260vs printer. ABS like substrates do not work since they remain porous. The final 3D printed products may need some light sanding, any sandpaper will do but we use 100 medium grit.
Protocols for brain sections using this chamber or others: https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/cpns.84